20x SSPE Buffer 1 L
20X SSPE is a prepared buffer concentrate for nucleic acid hybridizations and blot transfer applications.
20x SSPE Buffer 1 L
Nr produktu: 125665119

20x SSPE Buffer 1 L

Nr produktu: 125665119

PLN 260

Price Details

Excluding Shipping & Custom charges ( Shipping and custom charges will be calculated on checkout )

*All items will import from Stany Zjednoczone

w magazynie
Stany Zjednoczone Zaimportowano ze sklepu USA
Zamów teraz i otrzymaj około Piątek, Lipiec 10
Nasi czołowi partnerzy logistyczni
  • fedex
  • dhl
20X SSPE is a prepared buffer concentrate for nucleic acid hybridizations and blot transfer applications.
Gwarancja U-Care:
Nic
Wybierz plan
fast shipping

Fast
Shipping

free return

Free
Return*

Bezpieczne pakowanie

Bezpieczne pakowanie

100% oryginalne produkty

100% oryginalne produkty

pci-dss

PCI DSS Compliance

iso certified

ISO 27001 Certified


paypal payment
visa payment
mastercard payment
bank transfer payment
przelewy24 payment
blik payment
Note: Step Down Voltage Transformer required for using electronics products of Stany Zjednoczone store (110-120). Recommended power converters Kup Teraz.

Szczegóły Produktu

Shop 20x SSPE Buffer 1 L online at a best price in Poland. B0DJDXMV94
Item Weight2.2 lbs (1 kg)

OPIS PRODUKTU

20x SSPE Buffer 1 L

Masz jakieś zapytanie? Porozmawiaj z nami

Pytania i odpowiedzi klientów

  • pytanie: What is 20x SSPE Buffer and how is it used?

    odpowiedź: 20x SSPE Buffer is a high-concentration solution used primarily in molecular biology for various applications such as hybridization and washing of nucleic acid blots. This buffer provides an ideal ionic environment, enhancing the stability of DNA and RNA interactions during experiments. It's especially useful in procedures like Southern and Northern blotting, where high salt concentration helps maintain strand associations. By diluting it to a working concentration, researchers can optimize conditions for their experiments effectively.
  • pytanie: What are the components of 20x SSPE Buffer?

    odpowiedź: 20x SSPE Buffer typically consists of sodium chloride, sodium phosphate, and EDTA. These components are crucial for maintaining the appropriate ionic strength and pH conditions during molecular biology applications. Sodium chloride stabilizes nucleic acid interactions, while sodium phosphate adjusts the pH, allowing for optimal hybridization conditions. EDTA chelates divalent metal ions that could otherwise degrade nucleic acids, ensuring sample integrity for accurate results.
  • pytanie: How should 20x SSPE Buffer be stored?

    odpowiedź: 20x SSPE Buffer should be stored at a temperature of 4°C for short-term use and can be frozen at -20°C for long-term storage. When storing, it’s advisable to use airtight containers to prevent contamination and degradation from moisture and light. Regularly checking for any signs of precipitation or discoloration can ensure the buffer remains effective for experiments. Proper storage will help maintain its stability and reliability in various laboratory applications.
  • pytanie: Can 20x SSPE Buffer be used for DNA gel electrophoresis?

    odpowiedź: While 20x SSPE Buffer is primarily designed for hybridization applications, it is not typically used as a running buffer for DNA gel electrophoresis. Instead, running buffers like TAE or TBE are preferred, as they provide better separation capabilities for nucleic acids. However, SSPE can be useful in post-electrophoresis applications, such as in southern blotting, to help maintain DNA stability during the transfer and hybridization processes.
  • pytanie: What dilution is required to prepare a working solution from 20x SSPE Buffer?

    odpowiedź: To prepare a working solution from 20x SSPE Buffer, dilution to a 1x concentration is required. This means mixing one part of the 20x buffer with 19 parts of distilled water or an appropriate diluent. For example, to create 100 mL of 1x SSPE, you would add 5 mL of 20x buffer to 95 mL of water. This diluted solution can then be used in various downstream applications, enhancing the effectiveness of nucleic acid assays.
  • pytanie: Is 20x SSPE Buffer suitable for use in RNA experiments?

    odpowiedź: Yes, 20x SSPE Buffer is suitable for RNA experiments and is particularly useful in applications such as RNA blotting and hybridization. Its high salt concentration helps maintain RNA stability and prevents the degradation of RNA during experimental procedures. In scenarios where high specificity in hybridization is essential, using 20x SSPE can enhance the signal and reduce background noise by providing optimal ionic conditions for RNA binding.
  • pytanie: What role does EDTA play in 20x SSPE Buffer?

    odpowiedź: EDTA in 20x SSPE Buffer serves as a chelating agent that binds divalent metal ions like magnesium and calcium, which can catalyze the degradation of nucleic acids. By sequestering these ions, EDTA enhances the stability of both DNA and RNA during laboratory procedures, minimizing the risk of enzymatic breakdown. This is particularly important in applications like hybridization, where the integrity of nucleic acids is crucial for reliable results.
  • pytanie: How can you utilize 20x SSPE Buffer in PCR applications?

    odpowiedź: While 20x SSPE Buffer is not typically used directly in PCR, it can be beneficial in the post-amplification steps, such as purification and probe hybridization. It provides a stable ionic environment for maintaining the integrity of PCR products. After amplification, using a 1x dilution of SSPE can help in the downstream applications, ensuring that the amplified fragments remain stable and intact during further analysis.
  • pytanie: Where can I buy 20x SSPE Buffer 1 L in Poland?

    odpowiedź: You can buy 20x SSPE Buffer 1 L from Ubuy. They offer a variety of laboratory supplies, including this specific buffer, making it a reliable choice for your research needs in Poland.

Buffers Editorial Review

Nie znaleziono recenzji redakcyjnych

Opinie i oceny klientów

5.0
1 oceny klientów
  • 5 gwiazdka
    100%
  • 4 gwiazdka
    0%
  • 3 gwiazdka
    0%
  • 2 gwiazdka
    0%
  • 1 gwiazdka
    0%

Zrecenzuj ten produkt

Podziel się opinią z innymi klientami

Historia ceny produktu

Ważne informacje

  • Ograniczenia: W przypadku produktów wysyłanych za granicę należy pamiętać, że wszelkie gwarancje producenta mogą być nieważne; opcje serwisowe producenta mogą być niedostępne; instrukcje obsługi produktów, podręczniki i ostrzeżenia dotyczące bezpieczeństwa mogą nie być dostępne w języku kraju docelowego; produkty (i materiały towarzyszące) mogą nie spełniać norm wykonania, specyfikacji i wymogów dotyczących oznaczeń kraju docelowego; produkty mogą nie spełniać standardów dotyczących napięcia i innych norm elektrycznych kraju docelowego (co w stosownych przypadkach wymaga użycia adaptera lub konwertera). Odbiorca jest odpowiedzialny za sprawdzenie, czy produkt może zostać legalnie zaimportowany do kraju docelowego. W przypadku zamawiania produktów w sklepie Ubuy lub u jego partnerów odbiorca jest oficjalnie zgłoszonym importerem i musi spełniać wszystkie wymogi przepisów oraz regulacji kraju docelowego.
  • Nie wszystkie produkty znajdujące się na Ubuy są na sprzedaż, ponieważ Ubuy to globalna wyszukiwarka. Produkty podlegają przepisom eksportowym/handlowym.